Incorporation of Selenomethionine into Proteins through Selenohomocysteine-Mediated Ligation

Incorporation of selenomethionine into proteins through selenohomocysteine-mediated ligation.

Incorporation of Se75-selenomethionine into Pancreatic Juice Proteins in Vivo.

Selenomethionine Incorporation into Amyloid Sequences Regulates Fibrillogenesis and Toxicity

BACKGROUND The capacity of a polypeptide chain to engage in an amyloid formation process and cause a conformational disease is contained in its sequence. Some of the sequences undergoing fibrillation contain critical methionine (Met) residues which in vivo can be synthetically substituted by selenomethionine (SeM) and alter their properties. METHODOLOGY/PRINCIPAL FINDINGS Using peptide synthe...

In vitro incorporation of selenomethionine into protein by astragalus polysomes.

Selenium-accumulator plants synthesize selenium compounds that differ from those produced by nonaccumulators. To determine if there are any subcellular differences between accumulators and nonaccumulators in the use of selenomethionine in vitro, polysomes from Astragalus crotalariae (accumulator) and Astragalus lentiginosis (nonaccumulator) were translated in the presence of selenomethionine. P...

Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation.

The introduction of chemically unique groups into proteins by means of non-natural amino acids has numerous applications in protein engineering and functional studies. One method to achieve this involves the utilization of a non-natural amino acid by the cell's native translational apparatus. Here we demonstrate that a methionine surrogate, azidohomoalanine, is activated by the methionyl-tRNA s...